Thermo Fisher, 15367014, Plasmid pBR322

Name: Plasmid pBR322
Brand: Thermo Fisher
SKU: 15367014

Quantity: 25 μg, Unit Size: Each

Catalog Number: 15367014

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Category

Restriction Enzymes

Pack Size25 µg

TagsPlasmid DNA Biochemical Reagents DNA Reagents

CollectionsThermo Fisher Biochemical Reagents for Buffers, Stains & Core Lab Work Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 25 μg
Unit Size: Each
Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B E. coli, also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites for Hind III and Cla I are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance. Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation patterns are verified by agarose gel analysis. Transformation of E. coli HB101 to confirm AmpR and TetR is confirmed by plating on selective medium.Cloning, Restriction Enzyme Cloning

Specifications

General

Cloning Method	Restriction Enzyme/ MCS
Concentration	0.25 μg/μl
For Use With (Application)	Cloning
Product Type	Plasmid
Quantity	25 μg
Shipping Condition	Approved for shipment on Wet or Dry Ice
Vector	pBR322
Unit Size	Each

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Thermo Fisher, 15367014, Plasmid pBR322

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