Thermo Fisher, 15519028, Bluo-Gal

Quantity: 1 g
Unit Size: Each
Bluo-Gal (halogenated indolyl-β-galactoside) is an alternative histochemical substrate for β-galactosidase that produces a darker blue color than X-gal, the more frequently used substrate. As with X-gal, Bluo-Gal produces blue (lac+) bacterial colonies and blue (lac+) viral plaques. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.Bluo-Gal can also be used as a substrate with the streptavidin-β-galactosidase conjugate in both immunoblot and immunocytochemistry assays. In the presence of β-galactosidase, Bluo-Gal yields a blue precipitate, which is insoluble in alcohols and xylenes. Bluo-Gal is soluble in dimethylformamide.Primary applications• Blue-white screening (deeper blue as compared to X-gal)—detect functional β-galactosidase (reporter gene expression)• Histochemical detection of β-galactosidase activity in fixed tissues• Bluo-Gal overlay assayFind the media format that fits your needs• Ready-to-use media formulations are available, such as LB Broth (Cat. No. 10855001), Terrific Broth (Cat. No. A1374301), or S.O.C recovery medium (Cat. No. 15544034).• IPTG (Cat. No.15529019) is available for blue-white screening.