Thermo Fisher, 16726-252130, Accucore™ 150 Amide HILIC HPLC Columns

Accucore™ 150 Amide is a solid-core HILIC column for high-resolution separation of hydrophilic analytes and biomolecules. This analytical configuration measures 2.1 × 250 mm and contains 2.6 µm solid-core particles with a 150 Å pore size and amide stationary phase. The column is particularly well suited to glycan separations and other polar compounds that are poorly retained by conventional reversed-phase methods. It supports UHPLC operation up to 14,500 psi, a pH range of approximately 2–9 and temperatures up to 60 °C. The long 250 mm bed provides high resolving power while the solid-core particle design limits backpressure relative to similarly efficient sub-2 µm fully porous columns. The 2.1 mm internal diameter also reduces mobile-phase consumption compared with wider analytical formats. For glycan work, retention and selectivity are strongly influenced by water content, buffer concentration and column temperature, so equilibration should be consistent before comparing samples.