Thermo Fisher, 17100017, Collagenase, Type I, powder, 1 g

Name: Collagenase, Type I, powder, 1 g
Brand: Thermo Fisher
SKU: 17100017

Quantity: 1 g, Unit Size: Each

Catalog Number: 17100017

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Category

Collagenase Solutions

Pack Size125 units / pack

TagsCell Culture Media Supplements and Reagents Cell Culture Reagents Antibiotics and Supplements Dissociation Reagents

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 g
Unit Size: Each
• Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Gly-Pro, which is found with high frequency in collagen
• It is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone
• Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium
• Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment
• Gibco™ Collagenase Type I is isolated fromClostridium histolyticumand packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation, and organ perfusions
• Compared to other collagenase preparations, Gibco™ Collagenase Type I has average levels of collagenase, caseinase, clostripain, and tryptic activities, and is well-suited for the digestion of fat, adrenal, and liver cells or tissues
• Gibco™ Collagenase Type I activity is guaranteed to be greater than 125 units/mg
• Store at 2°C to 8°C, protected from light

Specifications

General

Quantity	1 g
Shelf Life	24 Months
Shipping Condition	Room Temperature
Form	Lyophilized
Product Type	Collagenase
Sterility	Non-sterile
Unit Size	Each

FAQs

Question: What is the supplied form?

Answer:The product is a lyophilized collagenase powder.

Question: What package size is supplied?

Answer:This configuration contains 1 g.

Question: How should it be stored?

Answer:Store at 2–8 °C and protect from light.

Application Note

Gibco™ Collagenase Type I is a lyophilized protease preparation used for enzymatic dissociation of collagen-rich tissues. Collagenase cleaves peptide bonds found frequently in collagen and can degrade native triple-helical collagen fibrils in connective tissues such as skin, tendon, blood vessels, and bone. Type I collagenase is used in tissue disaggregation workflows for primary cell isolation and cell-culture preparation, including a range of epithelial, kidney, brain, and tumor tissues. The enzyme is relatively gentle under physiological temperature and pH conditions, helping release cells without the extensive mechanical disruption required by some other methods. This configuration contains 1 g of non-sterile lyophilized powder. Store at 2–8 °C and protect from light. Prepare working solutions at concentrations validated for the tissue type and desired dissociation endpoint.

Ordering

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