Thermo Fisher, 17502048, N-2 Supplement (100X)

Name: N-2 Supplement (100X)
Brand: Thermo Fisher
SKU: 17502048

Quantity: 5 mL, Unit Size: Each, Gibco N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein's N-1 formulation, containing optimized concentrations of insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite. N-2 Supplement is recommended for growth and expression of neuroblastomas and neural cells in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS).

Catalog Number: 17502048

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Category

Lipid Supplements

Pack Size5 mL

TagsCell Culture Media Supplements and Reagents Cell Culture Reagents Antibiotics and Supplements Additional Cell Culture Reagents and Supplements

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 5 mL
Unit Size: Each
N-2 Supplement has been demonstrated in wide-ranging applications, such as the culture of neural stem cells, the culture of the SH-SY5Y cell line (subline of the neuroblastoma cell line SK-N-SH), and culture of the HT22 mouse hippocampal neuronal cell line. Additionally, N-2 Supplement adds support to cell culture media used to differentiate pluripotent stem cells (PSCs) to a variety of cell types, including neural and pancreatic-like cell types.N-2 Supplement may be used with Neurobasal media supplemented with growth factors such as bFGF and EGF. When combined with Dulbecco's Modified Eagle Medium (D-MEM) and fetal bovine serum (FBS), N-2 Supplement also provides suitable supplementation for the culture of human and rodent astrocytes.
• Each lot is cGMP manufactured
• Each lot is tested for sterility, endotoxin, and performance
• Contains insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite
• Can be used as a substitute for Bottenstein's N-1 formulation
• Provided as a 100X concentrateChoose Gibco N-2 Supplement for a reliable, high-quality product trusted by researchers worldwide.

Specifications

General

Cell Type	Neural Cells
Concentration	100 X
Culture Type	Mammalian Cell Culture
Quantity	5 mL
Serum Level	Serum-Free
Shelf Life	18 Months
Shipping Condition	Dry Ice
Tested For	Endotoxin, Performance, Sterility
Classification	Serum-free
Form	Liquid
Product Type	Cell Culture Supplement
Sterility	Sterile
pH	6 to 8
Unit Size	Each

FAQs

Question: Which components are present in N-2 Supplement?

Answer:It contains insulin, holo-transferrin, progesterone, putrescine and selenite.

Question: What working volume can the pack prepare?

Answer:The 5 mL pack is 100X and can prepare up to 500 mL at a final 1X concentration.

Question: What are the storage conditions?

Answer:Keep the supplement at −5 to −20°C protected from light.

Application Note

N-2 Supplement (100X) is designed for serum-free supplementation of neuronal and selected glial cell culture media. Its chemically defined formulation is based on the Bottenstein N-1 approach and contains insulin, iron-rich holo-transferrin, progesterone, putrescine and selenite. The supplement supports growth and expression of primary neuroblastoma cultures and postmitotic neurons derived from the peripheral or central nervous system. It may also support suitable astrocyte cultures when combined with an appropriate basal medium and other protocol components. Supplied as a sterile 5 mL liquid concentrate at 100X, it can produce up to 500 mL of medium when diluted to a final 1X concentration. This is the small-volume option and should not be confused with the separate 50 mL packaging. The product is tested for endotoxin, sterility and culture performance, making it useful for repeatable neuronal-cell experiments that need a defined, serum-free supplement. Store frozen at −5 to −20°C and protect from light; its shelf life is 18 months under the recommended conditions.

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