Thermo Fisher, 18297010, MAX Efficiency™ DH10B Competent Cells

Quantity: 5 x 200 μL
Unit Size: Each
MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency, making them ideal for generating plasmid libraries, an ability to take up and maintain large plasmids (seen with plasmids of 150 kb in size), and a lack of methylation-dependent restriction systems.Benefits of MAX Efficiency DH10B Competent Cells• Ability to maintain large DNA plasmids • Resistant to the effects of ligase and ligase buffer and tolerant of small amounts of undiluted ligation reactions • Suitable for the construction of cDNA or genomic DNA libraries using plasmid-derived vectors Universal strain for everyday applicationsThe presence of the mcrA genotypic marker and the deletion of mcrBC and mrr makes this strain suitable for cloning DNA that contains methylcytosine and methyladenine. Therefore, genomic DNA, both prokaryotic and eukaryotic, can be cloned efficiently in DH10B.Versatile DH10B genotype features• lacZ∆M15 for blue/white screening of recombinant clones • Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries • endA1 mutation to increase plasmid yield and quantity GenotypeF–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) nupGFind the strain and format that fits your need • DH10B Cells are available in both electrocompetent and chemically competent formats.• MegaX DH10B T1^R Electrocomp Cells (Cat. No.C640003) have the highest transformation efficiency (>3 x 10^10 cfu/μg plasmid DNA).• Multishot formats are available for high throughput applications.