Thermo Fisher, 194343001L, CaptureSelect™ tPA Affinity Matrix

Quantity: 1 L
Unit Size: Each
Features of this affinity matrix include:
• Neutral pH elution with 20 mM Tris, 2.0 M MgCl_2, 0.2 M arginine pH 7.0
• Caustic cleaning with 50 mM NaOH
• Excellent scalability
• Non-animal-derivedFree of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).Main characteristics:
• Matrix:agarose-based, epoxide-activated
• Average particle size:65 ± 10 μm
• Ligand:CaptureSelect tPA affinity ligand
• Ligand coupling method:aldehyde coupling via NH_2residues of the ligand
• Binding capacity:8-10 g/L depending on flow rate, column height, and contact time
• Elution conditions:Neutral pH: 20 mM Tris, 2.0 M MgCl_2, 0.2 M arginine pH 7.0; Low pH: 0.1 M glycine, pH 3.0
• Flow characteristics:50–200 cm/h (up to 2 bar)
• Formulation buffer:20% (v/v) ethanol