Thermo Fisher, 1943452010, CaptureSelect™ Prothrombin Affinity Matrix

Quantity: 10 mL
Unit Size: Each
Prothrombin is a vitamin-K dependent plasma protein which is produced in the liver. The presence of calcium promotes the binding of prothrombin to phospholipid bilayers.Features of this affinity matrix include:
• Highly selective for human prothrombin without cross reactivity to other human plasma proteins
• Binds different forms of prothrombin (Factor II) in the presence of calcium
• Enables efficient purification of prothrombin with high yields and retained activity
• Mild elution at neutral pH using EDTA
• Excellent scalability
• Non-animal-derivedFree of animal componentsCaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).Main characteristics:
• Matrix:agarose-based, epoxide activated
• Average particle size:65 ± 10 μm
• Ligand:CaptureSelect prothrombin affinity ligand
• Ligand coupling method:epoxide coupling
• Binding capacity:estimated at > 5 mg/mL
• Binding conditions:50 mM Tris, 150 mM NaCl, 5 mM CaCl_2, pH 7
• Elution conditions:50 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7
• Flow characteristics:50–200 cm/h (up to 2 bar)
• Formulation buffer:20% (v/v) ethanol