Thermo Fisher, 20195, Staphylococcus aureus V-8 Protease

Quantity: 5 mg
Unit Size: Each
Has two pH optima, one at pH 4.0 and the other at pH 7.8 and is specific for cleavage at the carboxy terminus of glutamic acid and aspartic acid residues. The protease is further specific for only glutamic digestion in buffers, which do not contain phosphate at either pH optimum. Phosphate buffers at pH 7.8 will facilitate digestion at both glutamic and aspartic acid residues. Highlights:
• Specificity for glutamic acid is achieved in ammonium bicarbonate, pH 7.8 or ammonium acetate, pH 4
• Enzyme is active in the presence of many denaturing agents such as SDS, urea and guanidine·HCl