Thermo Fisher, 21010046, Basal Medium Eagle (BME)

Name: Basal Medium Eagle (BME)
Brand: Thermo Fisher
SKU: 21010046

Quantity: 500 mL, Unit Size: Each

Catalog Number: 21010046

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Category

Classical Cell Culture Media

Pack Size500 mL

TagsCell Culture Media Supplements and Reagents Classical and Basal Cell Culture Media BME Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 mL
Unit Size: Each
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.This BME is manufactured as follows:
• With:Phenol Red
• Without:L-glutamine, HEPESThe complete formulation is available.BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO_2 incubator.BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO_2 environment to maintain physiological pH.

Specifications

General

Cell Line	HeLa, mouse fibroblasts, WI-38 and MRC-5
Concentration	1 X
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco
Product Type	Basal Medium Eagle (BME)
Quantity	500 mL
Shelf Life	12 Months From Date of Manufacture
Shipping Condition	Room Temperature
Classification	Animal Origin-free
Form	Liquid
Sterility	Sterile-filtered
With Additives	Low Glucose
Without Additives	No Glutamine
Unit Size	Each

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Thermo Fisher, 21010046, Basal Medium Eagle (BME)

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