Description
Quantity: 3.75 L
Unit Size: Each
These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems. The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer. Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG. Highlights:
• Three optimized binding buffers– for Protein A, Protein G or Protein A/G
• Two elution buffer options– acidic (pH 2.8) or high salt (near-neutral)
• Special system for mouse IgG1– selective, two-stage elution from Protein A agarose Item-Specific Features:
• Protein A IgG Binding Buffer:pH 8.0; phosphate-based; contains glycerol and EDTA
• Protein A/G IgG Binding Buffer:pH 8.0; phosphate-based; contains EDTA
• Protein G IgG Binding Buffer:pH 5.0; contains 0.02% sodium azide
• IgG Elution Buffer:pH 2.8; amine-based
• Gentle Ag/Ab Binding Buffer:pH 8.0; phosphate-free; contains 0.05% sodium azide
• Gentle Ag/Ab Elution Buffer:pH 6.6; high salt (ionic strength)
• Mouse IgG1 Mild Elution Buffer:pH 6.0; contains 0.02% sodium azide Includes: Kits contain both binding and elution buffers
