Description
Quantity: 200 mL
Unit Size: Each
This two-reagent kit is simple to use. Mix the two reagents (1:1 ratio) and immerse a developed piece of film (recent or old) in the working solution. Watch for 1 to 5 minutes until the desired image appears, then stop the reaction by rinsing the film in water. Pierce Background Eliminator for Film evenly reverses development-signal over the entire film, preserving relative densitometry values so that analysis and publication of results is still valid and possible. Highlights:
• For overexposed X-ray film– reverses development of silver-based autoradiography film removing silver emulsion from the film surface
• Recovers and preserved data– evenly reduces developed signal over the entire film so relative intensity (densitometry) of bands is not altered
• Optimizes for signal-to-noise– allows readjustment of overdeveloped film to obtain the best image with respect to background haze, speckles and oversaturated bands that need to be resolved
• Saves time and resources– eliminates the need to repeat experiments or re-expose a new piece of film to obtain an optimal image Recommended for:Remove background from any application that uses X-ray film exposures including: Western, Northern and Southern blots that use Thermo Scientific SuperSignal Substrates and the Pierce Chemiluminescent Hybridization and Detection Kit; In-gel detection systems; Gel-shift assays; Ribonuclease protection assays (RPA)
