Thermo Fisher, 21127030, Ham's F-12K (Kaighn's) Medium, 10 x 500 mL

Quantity: 10 x 500 mL
Unit Size: Each
Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.This F-12K (Kaighn's) Medium is manufactured as follows:With: L-glutamine, Phenol RedWithout: HEPESThe complete formulation is available.Using Ham's F-12KHam's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO_2 environment to maintain physiological pH.