Thermo Fisher, 21221, Pierce™ Avidin, Fluorescein (FITC) Conjugated

Quantity: 5 mg
Unit Size: Each
Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates. Highlights:
• Native avidin– glycoprotein from chicken egg whites, 67kDa, pI equals 10
• Soluble– glycosylation and isoelectric point renders avidin more soluble than streptavidin
• Affordable– significantly less expensive than recombinant streptavidin
• ABC staining– avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)
• Pure protein and conjugates– choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays