Thermo Fisher, 21600069, DPBS, powder, no calcium, no magnesium, 10 L

DPBS, powder, no calcium, no magnesium, 10 L is designed for preparing Dulbecco’s phosphate-buffered saline for routine cell-culture handling. After reconstitution, DPBS provides a balanced isotonic salt environment used for washing cells before dissociation, transporting cells or tissues, diluting cell suspensions for counting and preparing reagents. This formulation contains no calcium or magnesium, making it useful when divalent cations could interfere with cell-dissociation steps or when chelators such as EDTA need to be rinsed through the culture without reintroducing calcium or magnesium. The dry powder format prepares 10 L of working solution, allowing laboratories to make a larger batch while avoiding shipment and storage of the full liquid volume. DPBS is not a growth medium and does not replace complete culture medium; its role is to maintain appropriate osmotic and ionic conditions during short handling and wash procedures. The calcium- and magnesium-free formulation is particularly common before trypsinization or other detachment methods in adherent cell culture. Prepare the powder using the required water volume and sterile handling required by the intended workflow. The powder format is convenient for laboratories that consume larger volumes of wash solution and prefer to prepare buffered saline locally. Because calcium and magnesium are omitted, the reconstituted solution avoids divalent-cation effects during many cell-washing and dissociation-related procedures.