Thermo Fisher, 22400089, RPMI 1640 Medium, HEPES, 500 mL

Name: RPMI 1640 Medium, HEPES, 500 mL
Brand: Thermo Fisher
SKU: 22400089

Quantity: 500 mL, Unit Size: Each

Catalog Number: 22400089

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Category

Classical Cell Culture Media

Pack Size500 mL

TagsCell Culture Media Supplements and Reagents Classical and Basal Cell Culture Media RPMI Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 mL
Unit Size: Each
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This RPMI is modified as follows:With: L-glutamine, HEPES, Phenol RedThe complete formulation is available.Using RPMIRPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B_12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO_2 environment to maintain physiological pH.

Specifications

General

Cell Line	HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type	Leukemic Cells
Concentration	1 X
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity	500 mL
Shelf Life	12 Months From Date of Manufacture
Shipping Condition	Room Temperature
Classification	Animal Origin-free
Form	Liquid
Serum Level	Standard Serum Supplementation
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	Glutamine
Without Additives	No Sodium Pyruvate
Unit Size	Each

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Thermo Fisher, 22400089, RPMI 1640 Medium, HEPES, 500 mL

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