Description
Quantity: 5 g
Unit Size: Each
A solution of this compound produces a measurable yellow-colored product when it reacts with sulfhydryls. Consequently, Ellman's Reagent is very useful as a sulfhydryl assay reagent because of its specificity for –SH groups at neutral pH, high molar extinction coefficient and short reaction time. Sulfhydryl groups may be estimated in a sample by comparison to a standard curve composed of known concentrations of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups may be quantitated by reference to the extinction coefficient of TNB. Highlights:
• Sulfhydryl detection– reacts quantitatively with free (reduced) sulfhydryl groups
• Colorimetric–yields colored product to enable spectrophotemetric measurement in tubes or microplates
• Proven assay method– well-characterized chemistry enables quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard
