| Form | Solid |
|---|---|
| Label Type | Blocking Groups |
| Label or Dye | Unreactive Group |
| Product Line | Pierce |
| Quantity | 25 g |
| Reactive Moiety | Maleimide |
| Solubility | Water |
| Chemical Reactivity | Thiol |
| Product Type | NEM |
| Spacer | Medium (10 to 30 Å) |
| Unit Size | Each |
Answer:NEM reacts primarily with sulfhydryl groups such as reduced cysteine thiols.
Answer:This configuration contains 25 g.
Answer:Store the solid reagent at −20 °C.
Pierce™ N-ethylmaleimide (NEM) is a thiol-reactive alkylating reagent used to permanently block sulfhydryl groups such as reduced cysteine residues. The maleimide group forms a stable, nonreversible thioether bond with thiols, helping prevent reformation of disulfide bonds during protein and enzyme studies. This configuration contains 25 g of solid NEM. Reaction selectivity is highest for sulfhydryls around pH 6.5–7.5; at higher pH, reaction with amino groups becomes more significant. NEM can be used to cap free thiols, inhibit sulfhydryl-dependent enzymes, or prevent thiol exchange during protein characterization. Reaction progress can be followed by the decrease in absorbance near 300 nm as the maleimide double bond is consumed. The compound is water soluble and should be stored at −20 °C. Prepare working solutions at concentrations appropriate for the target protein or assay.
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