Thermo Fisher, 23221, Pierce™ BCA Protein Assay Reagent A, 250 mL

Quantity: 250 mL
Unit Size: Each
• A component of the Pierce BCA Protein Assay Kit
• Kit is a two-component, high-precision, detergent-compatible assay that is used for total protein concentration determination compared to a protein standard
• Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research
• The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications
• Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy
• Estimate visually or measure with a standard spectrophotometer or plate reader at 562 nm
• Exhibits less protein-to-protein variation than dye-binding methods (Bradford)
• Unaffected by typical concentrations of most ionic and non-ionic detergents
• Assay has a 30 min. incubation; much easier and four times faster than classical Lowry methods
• Linear working range for BSA of 20 to 2000 μg/mL
• Detect down to 5 μg/mL with the enhanced protocol
• Store at room temperature