Thermo Fisher, 23400021, RPMI 1640 Medium, powder, HEPES

Name: RPMI 1640 Medium, powder, HEPES
Brand: Thermo Fisher
SKU: 23400021

Quantity: 10 x 1 L, Unit Size: Each, Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications.

Catalog Number: 23400021

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Category

Classical Cell Culture Media

TagsCell Culture Media Supplements and Reagents Classical and Basal Cell Culture Media RPMI Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 x 1 L
Unit Size: Each
This RPMI is modified as follows:With: L-glutamine, Phenol Red, HEPESWithout: Sodium BicarbonateThe complete formulation is available.Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B_12 and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.Product Intended UseFor Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO_2 environment to maintain physiological pH.Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

Specifications

General

Cell Line	HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type	Leukemic Cells
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco
Product Type	RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity	10 x 1 L
Shelf Life	36 Months From Date of Manufacture
Shipping Condition	Room Temperature
Classification	Animal Origin-free
Form	Powder
Serum Level	Standard Serum Supplementation
Sterility	Sterile-filtered
With Additives	HEPES
Without Additives	No Sodium Bicarbonate
Unit Size	Each

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Thermo Fisher, 23400021, RPMI 1640 Medium, powder, HEPES

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