Thermo Fisher, 25005-254630, Hypersil GOLD™ C18 Selectivity HPLC Columns

Name: Hypersil GOLD™ C18 Selectivity HPLC Columns
Brand: Thermo Fisher
SKU: 25005-254630

Length (Metric): 250 mm, Diameter (Metric): 4.6 mm, Particle Size: 5 μm, Unit Size: Each, Achieve exceptional peak shape and resolution for your HPLC and LC/MS applications with Thermo Scientific™ Hypersil GOLD™ HPLC columns. These endcapped, ultrapure, silica-based columns deliver significant reduction in peak tailing using generic gradients with C18 selectivity. With their excellent resolution, efficiency, and sensitivity, Hypersil GOLD columns give you confidence in the accuracy and quality o

Catalog Number: 25005-254630

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Category

Reversed-Phase HPLC Columns

TagsHPLC Columns Chromatography Columns and Cartridges HPLC Columns and Cartridges

CollectionsThermo Fisher Chromatography Columns & Cartridges (HPLC/UHPLC, GC, IC, SPE)Thermo Fisher Chromatography Columns & Guard Cartridges (HPLC, UHPLC, GC)Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs

Description

Length (Metric): 250 mm
Diameter (Metric): 4.6 mm
Particle Size: 5 μm
Unit Size: Each
Novel Bonding
• Proprietary derivatization and endcapping
• C18 (USP L1) selectivity pH Stability
• Robust performance at high pH
• Excellent column stability at low pH Flexible Formats
• Javelin HTS columns for high-throughput screening
• Semi-preparative columns with 12 μm particles available About the Hypersil GOLD Family of HPLC Columns Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different column chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.
• Excellent peak symmetry
• Narrow peaks for outstanding efficiency
• Enhanced sensitivity and improved resolution
• Exceptional reproducibility for reliable chromatography, column after column
• Solutions for high-throughput screening
• 1.9, 3, and 5 μm particle sizes for all your separation needs

Specifications

General

Column Format	Analytical Column
Column Type	Reversed Phase
Endcapped	Yes
Max. Pressure	5800 psi (400 bar)
Packing Material	Spherical, Fully Porous, Ultrapure Silica
Quantity	Each
Stationary Phase	C18 Selectivity
Surface Area	220 m²/g
Temperature	60°C
USP Type	L1
Carbon Load	11%
Diameter (Metric)	4.6 mm
Length (Metric)	250 mm
Particle Size	5 μm
Phase	Reversed Phase
Pore Size	175 Å
Product Line	Hypersil GOLD
pH	1 to 11
Unit Size	Each

FAQs

Question: What are the column dimensions and particle size?

Answer:The column is 250 mm × 4.6 mm and contains 5 µm fully porous spherical silica particles.

Question: What pH range can this column operate in?

Answer:The operating pH range is 1–11.

Question: What are the maximum temperature and pressure?

Answer:The maximum temperature is 60 °C and the maximum pressure is 400 bar (5800 psi).

Application Note

Hypersil GOLD™ C18 Selectivity HPLC Columns is designed for reversed-phase HPLC and LC-MS separations that require C18 selectivity and controlled silanol activity. This configuration is 250 mm long with a 4.6 mm internal diameter and is packed with 5 µm fully porous spherical silica. The endcapped C18 phase has a 175 Å pore size, a surface area of 220 m²/g and an 11% carbon load and corresponds to USP L1. The column operates across pH 1–11 and at temperatures up to 60 °C, with a maximum pressure of 400 bar (5800 psi). High-purity silica and the bonded surface chemistry support symmetrical peak shapes and reduced tailing across a broad range of analytes. The 250 mm analytical format provides a long separation bed for methods that require resolving power while retaining a conventional 4.6 mm HPLC internal diameter. It can be used for method development, routine quantitative analysis and LC-MS workflows when the separation requires this C18 phase and the stated pH, temperature and pressure range.

Ordering

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