Thermo Fisher, 25300054, Trypsin-EDTA (0.05%), phenol red, 100 mL

Gibco™ Trypsin-EDTA 0.05% is a cell-dissociation reagent for routine passaging of attachment-dependent mammalian cells. This 100 mL formulation contains porcine pancreatic trypsin together with EDTA and phenol red. Trypsin cleaves cell-surface and extracellular-matrix proteins that mediate attachment, while EDTA chelates calcium and magnesium ions that help stabilize cell-cell and cell-matrix interactions. The lower 0.05% trypsin concentration is widely used for cell lines that detach readily or where a milder dissociation condition is preferred. Phenol red provides a visual pH indicator during handling. Exposure time should be controlled for the specific cell type because prolonged protease treatment can reduce viability or damage surface proteins. After detachment, trypsin activity is typically neutralized using serum-containing medium or another validated inhibitor before cells are collected and reseeded.