Thermo Fisher, 26179, Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse

Name: Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse
Brand: Thermo Fisher
SKU: 26179

Quantity: 10 Tests, Unit Size: Each, Thermo Scientific™ Pierce Rapid Mouse Antibody Isotyping Kits are single-use, cassette-based kits for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity.

Catalog Number: 26179

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Category

Western Blot Detection Reagents

Pack Size10 tests

TagsProtein Analysis Reagents Antibody Production and Purification Antibody Isotyping and Characterization

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 Tests
Unit Size: Each
The kits are composed of small cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well. The kits determine mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM.The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.Highlights:
• Long shelf life– stable for more than 1 year at room temperature
• Single-step– add diluted antibody sample to the loading-well of cassette
• Fast– within 5 minutes a band appears that indicates the antibody isotype
• Sensitive– detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10ng/mL
• Specific– assay does not crossreact with fetal bovine serum (FBS)
• Reliable– results are similar to the standard ELISA-based isotyping assays Includes:Ten pouches, each containing two (Part No. 26178) or three (Part No. 26179) cassettes, plus a bottle of sample diluent

Specifications

General

Description	Pierce Rapid Isotyping Kit plus Kappa and Lambda - Mouse
For Use With (Application)	Membrane Blot
Quantity	10 Tests
Assay	Isotyping Assay
Detection Method	Chromogenic
Product Line	Pierce
Target Specificity	Target-specific
Unit Size	Each

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