Description
Quantity: 8 x 250 μL
Unit Size: Each
The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped for detection of near-infrared (NIR) fluorescence. The bands are also directly visible because the proteins are prestained blue. The 55kDa band is of greater intensity and serves as a reference band. The protein ladder is conveniently packaged and ready to use: no heating, dilution or addition of a reducing agent is required before use.Highlights:
• Size range10 proteins spanning 11 to 250kDa
• Fluorescentdetect using 670nm red laser or 700nm channels with fluorescence imagers
• Ready-to-usesupplied in a loading buffer for direct loading on gels
• Sharp bandsexcellent sizing for near-infrared visualization of protein gels and blots
• Reference band55kDa band has greater intensity for easy orientation
• Quality testedeach lot evaluated by SDS-PAGE and Western blottingIncludes:Proteins in 62.5mM Tris-H_3PO_4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN_3 and 33% glycerolRequires:Fluorescent imager capable of near-infrared fluorescence detection, such as certain Typhoon™ Imagers and the LI-COR Odyssey™ Infrared Imaging SystemRecommended for:Protein sizing on SDS-polyacrylamide gels and Western blots for visualization with near-infrared (NIR) fluorescence imagers