Thermo Fisher, 26637, PageRuler™ Unstained High Range Protein Ladder

Quantity: 2 x 250 μL
Unit Size: Each
The protein MW markers in this ladder resolve into clearly identifiable, sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.Highlights:
• Size range8 proteins spanning 60 to 250kDa
• Ready-to-usesupplied in a loading buffer for direct loading on gels
• Sharp bandsexcellent accuracy for detection with coomassie or silver stains
• Reference band150kDa band has greater intensity for easy orientation
• Taggedeach protein in the ladder contains an integral Strep-tag™ II Sequence
• Quality testedeach lot evaluated by SDS-PAGE and Western blottingIncludes:
• Proteins (0.02 to 0.05mg/mL of each) in 62.5mM Tris-H_3PO_4(pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN_3, 0.01% bromophenol blue and 33% glycerolRecommended for:
• Accurate protein sizing on SDS-polyacrylamide gels and Western blots