Thermo Fisher, 27-1794-10, Phosphorylating Phosphoramidite, standard grade, 28-400 finish bottle

Quantity: 0.5 g
Unit Size: Each
Phosphorylating phosphoramidite can be utilized to achieve phosphorylation of the 5' oligo terminus (also known as Phosphate-ON or Chemical Phosphorylation Reagent (CPR)). Chemical phosphorylation is a cost-effective substitute to enzymatic methods (via T4 kinase and ATP), allowing efficient introduction of terminal phosphate groups. Oligos with a 5'-phosphate are conducive to a number of various applications utilizing the ligation of oligos to each other, such as NGS adapters, cloning, and gene construction. This product is supplied in a 30 mL amber bottle with a 28-400 Finish neck style, which is compatible with most MerMade Systems of oligonucleotide synthesizers. Features:• Full chemical name: 2-[2-(4,4-Dimethoxytrityloxy)ethylsulfonyl]ethyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite• Molecular formula: C_34H_45F_3N_2O_7PS• Formula weight: 656.66 g/mol• HPLC Purity: ≥95.0%