Thermo Fisher, 28010, HABA (4'-hydroxyazobenzene-2-carboxylic acid)

Name: HABA (4'-hydroxyazobenzene-2-carboxylic acid)
Brand: Thermo Fisher
SKU: 28010

Quantity: 10 g, Unit Size: Each, Thermo Scientific™ Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.

Catalog Number: 28010

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Category

Protein Biotinylation Kits

Pack Size10 g

TagsProtein Analysis Reagents Crosslinking Labeling and Protein Modification Protein Labeling Reagents

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 g
Unit Size: Each
Thermo Scientific Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules. Determine the molar ratio of biotin incorporated into a protein using the HABA-Avidin method. The HABA dye (4'-hydroxyazobenzene-2-carboxylic acid ) binds to avidin to produce a yellow-orange colored complex which absorbs at 500nm. Free biotin will displace the HABA dye and cause the absorbance to decrease. A standard curve can be established using the free biotin to estimate the number of moles of biotin incorporated after biotinylating a protein.Highlights:
• HABA-avidin complex can be used over a wide range of pH and salt concentrations
• Amount of Avidin can be calculated directly from the increased absorbance at 500nm complexing with the HABA Dye
• Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions or use Pierce's online HABA calculator
• Complete kits also available! See Pierce Biotin Quantitation Kit (Part No. 28005) and Fluorescence Biotin Quantitation Kit (Part No. 46610)

Specifications

General

Product Line	Pierce
Product Type	HABA
Quantity	10 g
Labeling Target	Proteins
Label or Dye	Biotin
Unit Size	Each

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Thermo Fisher, 28010, HABA (4'-hydroxyazobenzene-2-carboxylic acid)

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