Thermo Fisher, 28205-254630, Hypersil™ BDS C8 HPLC Columns

Hypersil™ BDS C8 HPLC Column, 250 x 4.6 mm, 5 µm is used for reversed-phase HPLC when C8 selectivity and lower retention than a comparable C18 phase are preferred. The column uses a fully endcapped, base-deactivated C8 stationary phase on spherical, fully porous silica and is packed with 5 µm particles in a 4.6 mm inner-diameter × 250 mm analytical format. The Hypersil BDS C8 phase has a 130 Å pore size, 170 m²/g surface area, and 7% carbon load. It supports pH 2–9, temperatures up to 60 °C, and pressures up to 5800 psi (400 bar). Its reduced silanol activity and base-deactivated surface support acids, bases, and neutral compounds and make the phase well suited to routine QC/QA assays. The C8 chemistry provides less hydrophobic retention than C18, which can shorten analysis for compounds that do not require stronger C18 retention. This 250 mm column uses the exact 5 µm particle size and 4.6 mm internal diameter for methods that specify this Hypersil BDS C8 configuration.