Thermo Fisher, 31150022, Medium 199, Earle's Salts, 500 mL

Name: Medium 199, Earle's Salts, 500 mL
Brand: Thermo Fisher
SKU: 31150022

Quantity: 500 mL, With Additives: Glutamine, Unit Size: Each

Catalog Number: 31150022

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Category

Classical Cell Culture Media

Pack Size500 mL

TagsCell Culture Media Supplements and Reagents Classical and Basal Cell Culture Media Medium199 Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 mL
With Additives: Glutamine
Unit Size: Each
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This Medium 199 is modified as follows:With: L-glutamine, Phenol RedWithout: HEPESThe complete formulation is available.Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO_2 incubator, or with Hanks' salts for use without CO_2.Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO_2 environment to maintain physiological pH.

Specifications

General

Cell Type	Mammalian Cells
Concentration	1X
Culture Environment	CO<sub>2</sub>
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Osmolality	290 - 310 mOsm/kg
Product Line	Gibco
Product Type	Medium 199
Quantity	500 mL
Shelf Life	12 Months
Shipping Condition	Room Temperature
Classification	Animal Origin
Culture Type	Mammalian Cell Culture
Form	Liquid
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	Glutamine
Without Additives	No HEPES
pH	7.1 to 7.4
Unit Size	Each

Ordering

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Thermo Fisher, 31150022, Medium 199, Earle's Salts, 500 mL

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