Thermo Fisher, 31490, Pierce™ Horseradish Peroxidase, 10 mg

Name: Pierce™ Horseradish Peroxidase, 10 mg
Brand: Thermo Fisher
SKU: 31490

Quantity: 10 mg, Unit Size: Each, Thermo Scientific™ Pierce Horseradish Peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blotting and immunohistochemistry applications.

Catalog Number: 31490

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Category

Phosphorylation Reagents

Pack Size10 mg

TagsProtein Analysis Reagents Protein Detection Systems

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 10 mg
Unit Size: Each
Highlights:
• Superior to alkaline phosphatase and β-galactosidase conjugates due to the higher specific enzyme activity
• Small size (40kDa) allows excellent cellular penetration
• Variety of substrates available
• Ideal in blotting and cytochemistry applications
• Used as the reporter enzyme for Thermo Scientific SuperSignal Chemiluminescent Western Blotting and ELISA SubstratesNote:Porstmann, B., Porstmann, T., Nugel, E. and Evers, U. (1985). Which of the commonly used marker enzymes gives the best results in colorimetric and fluorimetric enzyme immunoassays: horseradish peroxidase, alkaline phosphatase, β-galactosidase, J. Immunol. Meth. 79, 27-37.Wordinger, R.J., Miller, G.W. and Nicodemus, D.S. (1987). Manual of Immunoperoxidase Techniques, 2nd Edition. Chicago: American Society of Clinical Pathologists Press, pp. 23-24.Yolken, R.H. (1982). Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects. Rev. Infect. Dis. 4(1), 35-68.Cordell, J.L., et al. (1984). Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J. Histochem. Cytochem. 32, 219-229.Passey, R.B., et al. (1977). Evaluation and comparison of 10 glucose methods and the reference method recommended in the proposed product class standard. Clin. Chem. 23(1), 131.Hosoda, H., Takasaki, W., Tsukamoto, R. and Nambara, T. (1987). Sensitivity of steroid immunoassays. Comparison of alkaline phosphatase, β-galactosidase and horseradish peroxidase as labels in a colorimetric assay system. Chem. Pharm. Bull. 35, 3336-3342.Samoszuk, M.K., et al. (1989). Antibody, Immunoconjugates and Radiopharmaceuticals 2, 37-46.

Specifications

General

Label or Dye	HRP (Horseradish Peroxidase)
Quantity	10 mg
For Use With (Application)	Western Blotting, ELISA, Immunohistochemistry
Form	Powder
Product Line	Pierce
Unit Size	Each

Ordering

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Thermo Fisher, 31490, Pierce™ Horseradish Peroxidase, 10 mg

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General

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