Thermo Fisher, 31700, Peptide Retention Standard

Name: Peptide Retention Standard
Brand: Thermo Fisher
SKU: 31700

Quantity: 1 Vial, Unit Size: Each, Thermo Scientific™ Pierce Peptide Retention Standard is a mixture of five c-terminal amide decapeptides that provide reference elution profiles in reverse-phase HPLC.

Catalog Number: 31700

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Category

Western Blot Detection Reagents

Pack Size1 vials

TagsProtein Analysis Reagents Amino Acid Analysis Reagents and Accessories

CollectionsThermo Fisher Protein Analysis Reagents for Western Blot, ELISA & Proteomics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 Vial
Unit Size: Each
Retention times are predicted by totaling the values that represent the contribution in minutes of each amino acid residue and the peptide terminal groups. Retention time is dependent upon the molecular weight of the peptide. The effect on retention is relatively unimportant with a small peptide, but it increases with the size of the molecule. The accuracy of predicting peptide retention time significantly decreases beyond 20 residues. To ensure accuracy, a peptide standard is used to correct for instrument variation, column aging, n-alkyl chain length variation and ligand density. Highlights:
• Save time in peptide purification
• Simplify identification of specific peptides in a complex mixture
• Increase the efficiency of predicting peptide elution profiles Determine the relative order of peptide elution of a complex mixture Predict the HPLC retention time for peptides of known amino acid composition on reverse phase HPLC columns Monitor column performance: efficiency, selectivity and resolution during column aging Compare reverse-phase columns from different manufacturers Evaluate reverse-phase supports of varying n-alkyl chain lengths and ligand densities

Specifications

General

For Use With (Equipment)	HPLC
Quantity	1 Vial
Workflow Step	Standardization
Detection Method	HPLC
Form	Solid
Product Type	Peptide Retention Standard
Starting Material	Peptides
Unit Size	Each

Ordering

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Unless otherwise stated, the minimum order amount is USD 1,000.
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Thermo Fisher, 31700, Peptide Retention Standard

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General

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