Thermo Fisher, 31985062, Opti-MEM™ I Reduced Serum Medium, 100 mL
Quantity: 100 mL, Unit Size: Each
Catalog Number: 31985062
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Category
Pack Size100 mL
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| Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
|---|---|
| Cell Type | Primary Fibroblasts |
| Concentration | 1 X |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Product Line | Gibco, Opti-MEM |
| Product Type | Opti-MEM I |
| Quantity | 100 mL |
| Shelf Life | 18 Months From Date of Manufacture |
| Shipping Condition | Room Temperature |
| Classification | Human Origin |
| Form | Liquid |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Phenol Red |
| Unit Size | Each |
Answer:One 100 mL bottle.
Answer:Yes. Both phenol red and L-glutamine are present.
Answer:Preparation of lipid-based transfection complexes and reduced-serum mammalian cell culture.
Gibco™ Opti-MEM™ I Reduced Serum Medium is a modified MEM formulation developed for mammalian-cell culture and lipid-mediated transfection workflows. This configuration contains one 100 mL sterile-filtered bottle, with L-glutamine and phenol red. Its formulation includes insulin, transferrin, hypoxanthine, thymidine, and trace elements that can support a reduction in serum supplementation in suitable cell lines. The medium is commonly used while forming lipid–nucleic acid transfection complexes and as an experimental culture medium for compatible adherent or suspension mammalian cells. It contains sodium bicarbonate buffering and is intended for use under an appropriate carbon-dioxide atmosphere. The product contains no fetal bovine serum, is stored at 2–8 °C protected from light, and is supplied for research or further manufacturing.
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