| Chemical Name or Material | Zwitterionic Organic Chemical Buffer |
|---|---|
| Color | Clear |
| Formulation | N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid |
| Recommended Storage | <b>Storage conditions:</b> 2°C to 8°C<br /><b>Shipping conditions:</b> Room temperature<br /><b>Shelf life:</b> 24 months from date of manufacture |
| Sterility | Sterile |
| Sterilization Method | Sterile-filtered |
| For Use With (Application) | Chromatin Biology |
| Grade | Biochemical |
| Physical Form | Liquid |
| Quantity | 100 mL |
| Solution Type | Buffer |
| Unit Size | Each |
Answer:1 M HEPES in a 100 mL bottle.
Answer:It helps stabilize pH, including during handling outside a CO₂ incubator.
Answer:Typically 10–25 mM, adjusted to the requirements of the medium and cells.
Gibco HEPES is a 1 M aqueous zwitterionic buffer solution used to increase pH stability in cell culture media and other biological preparations. HEPES provides buffering capacity near physiological pH and is particularly helpful during experimental steps conducted outside a carbon dioxide-controlled incubator. The 100 mL bottle contains concentrated HEPES solution with a pH specification of 7.2–7.5. Working concentrations commonly range from 10 to 25 mM, depending on the medium and the cell-culture protocol. This concentrated supplement is added to an existing formulation rather than used as a complete cell culture medium. Storage at 2–8 °C maintains the stock in its recommended liquid condition.
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