Thermo Fisher, 65-0842-85, eBioscience™ Cell Proliferation Dye eFluor™ 450, 500 μg

Name: eBioscience™ Cell Proliferation Dye eFluor™ 450, 500 μg
Brand: Thermo Fisher
SKU: 65-0842-85

Quantity: 500 μg, Unit Size: Each

Catalog Number: 65-0842-85

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Category

Cell Lysis Buffers

Pack Size500 µg

TagsCell Analysis Products Cell Based Assays Cell Function Reagents and Kits

CollectionsThermo Fisher Cell Analysis Products: Viability, Apoptosis, ROS & Calcium Imaging Assays Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 μg
Unit Size: Each
• A violet fluorescent dye that can be used to monitor individual cell divisions
• Dye binds to any cellular proteins containing primary amines, and as cells divide, the dye is distributed equally between daughter cells
• They can be measured as successive halving of the fluorescence intensity of the dye - visualize up to 7 generations
• Cells labeled with Cell Proliferation Dye eFluor™ 450 may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers
• Cell Proliferation Dye eFluor™ 450 has a peak excitation of 409 nm and can be detected using a 450/50 band pass filter (equivalent to eFluor™ 450 or Pacific Blue™), making it compatible with applications that utilize GFPAdditional Details
• Each vial may be reconstituted to a stock concentration of 10 mM with 165 μL of anhydrous DMSO
• Once reconstituted it should be used within 6 months and protected from light and stored at -20°C with dessicant
• Avoid freeze-thawing

Specifications

General

Detection Method	Fluorescence
Form	Lyophilized powder
Quantity	500 μg
Color	Violet
Emission	405/450
For Use With (Application)	Proliferation Assay
For Use With (Equipment)	Flow Cytometer
Product Line	eBioscience
Product Type	Dye
Unit Size	Each

FAQs

Question: What does the eFluor 450 proliferation dye measure?

Answer:Successive dilution of fluorescent labeling as stained cells divide, measured by flow cytometry.

Question: What excitation and detection optics are suitable?

Answer:Excitation near 409 nm and collection through a 450/50 nm band-pass filter.

Question: What format and quantity are supplied?

Answer:A 500 µg vial of lyophilized fluorescent dye for reconstitution before cell labeling.

Application Note

The Invitrogen eBioscience Cell Proliferation Dye eFluor 450 is an amine-reactive violet fluorescent tracer for following cell divisions by flow cytometry. The fluorescent label covalently binds to proteins containing primary amines and becomes distributed between daughter cells during proliferation, creating successive peaks of decreasing signal intensity. Its excitation maximum is approximately 409 nm, with detection using a 450/50 nm band-pass filter. The 500 µg vial contains lyophilized dye that can be reconstituted in anhydrous DMSO to prepare a labeling stock. The dye supports monitoring of up to seven cell generations under suitable assay conditions and can be used with downstream fixation and permeabilization workflows.

Ordering

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