Thermo Fisher, 32500035, DMEM/F-12, powder, 10 L

Gibco™ DMEM/F-12 combines Dulbecco’s Modified Eagle Medium with Ham’s F-12 nutrient mixture in a 1:1 basal formulation for mammalian cell culture. The 10 L powder package allows laboratories to prepare a larger volume of medium when needed for routine cell propagation and method development. This formulation contains L-glutamine and phenol red but does not contain HEPES or sodium bicarbonate in the powder. Medium preparation therefore includes the required sodium bicarbonate addition, pH adjustment and filtration before culture use. DMEM/F-12 provides amino acids, vitamins, glucose and inorganic nutrients, while appropriate cell types may require serum or other defined supplements. Applications include maintenance or expansion of fibroblasts, epithelial-derived cells and various other mammalian cell models. This is a dry medium powder rather than a sterile, ready-to-use liquid.