Thermo Fisher, 34018, 1-Step™ TMB-Blotting Substrate Solution

Quantity: 250 mL
Unit Size: Each
TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used. Highlights:
• HRP substrate– for sensitive detection of horseradish peroxidase activity on solid media
• Chromogenic– no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates
• Ready-to-use– no organic solvents are required to dissolve; no dilution necessary for use
• Complete– does not require other buffers or addition of hydrogen peroxide