| Conjugate | HRP |
|---|---|
| Kit Contents | Stable Peroxide Buffer, 100 mL |
| Recommended Antibody Concentrations | 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL) |
| Sensitivity | Picogram to high femtogram-level |
| Shipping Condition | Room Temperature |
| Signal Duration | 6 to 24 hr. |
| Sufficient For | 25 Mini-Gel Blots, 2000 cm<sup>2</sup> of Membrane |
| Detection Method | Chemiluminescence |
| For Use With (Application) | Western Blot |
| Form | Liquid |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Product Line | SuperSignal |
| Product Type | Substrate |
| Quantity | 200 mL |
| Substrate | Horseradish Peroxidase |
| Unit Size | Each |
Answer:The substrate is designed for detection of horseradish peroxidase, or HRP, on immunoblots.
Answer:It contains 100 mL of Luminol/Enhancer Solution and 100 mL of Stable Peroxide Solution.
Answer:The supplied volume is sufficient for about 2,000 cm² of membrane, equivalent to roughly 25 mini-gel blots.
SuperSignal™ West Pico PLUS Chemiluminescent Substrate is used to detect horseradish-peroxidase-labeled targets on Western blots. The luminol-based formulation generates a chemiluminescent signal when HRP catalyzes oxidation in the presence of peroxide, supporting sensitive detection from picogram to high-femtogram levels. This 200 mL configuration contains 100 mL of Luminol/Enhancer Solution and 100 mL of Stable Peroxide Solution, which are mixed for use. The supplied volume is sufficient for approximately 25 mini-gel blots or 2,000 cm² of membrane. The substrate is compatible with nitrocellulose and PVDF membranes and provides a signal that can persist for approximately 6–24 hours, supporting imaging flexibility. Room-temperature storage simplifies routine Western blot workflows and avoids frozen-reagent handling.
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