Description
Quantity: 1 L
Unit Size: Each
The color produced by DAB (3,3'-diaminobenzidine) can be intensified by the addition of metals such as nickel, copper, silver and cobalt that form more dense complexes upon reaction in HRP-peroxide substrate reactions. The Thermo Scientific Pierce Metal Enhanced DAB Substrate Kit optimizes this intensifying chemistry, producing an exceptionally sensitive colorimetric detection system for Western blot, dot-blot and immunohistochemistry applications. The near-black signal (precipitate) is easy to photograph. Highlights:
• Incredible sensitivity– 50 times more sensitive than the traditional DAB method and 30 times more sensitive than other metal-intensified versions of DAB
• Low background, high intensity– get a crisp dark brown-black precipitate and, even with the increased intensity, background is almost nonexistent
• Six-hour stability– working solution is stable for more than six hours at room temperature! Other DAB substrates must be used immediately
• Convenient– eliminates the need to procure numerous reagents from various sources Includes:DAB/Metal Concentrate (10X), 25mL; Universal Blocker Blocking Buffer in TBS, 250mL; Peroxidase Suppressor, 2 × 100mL; Stable Peroxide Buffer, 250mL; BupH Tris Buffered Saline, 4 packs; Surfact-Amps 20 (10% Tween-20), 10mL; Harris Modified Hematoxylin, 100mL; Mounting Medium, 60mL