Thermo Fisher, 42200030, IMDM, powder, 10 L
Quantity: 10 L, Unit Size: Each
Catalog Number: 42200030
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Category
Pack Size10 L
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| Cell Type | Mammalian Cells |
|---|---|
| Culture Environment | CO<sub>2</sub> |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Osmolality | 215 - 245 mOsm/kg |
| Product Line | Gibco |
| Product Type | Iscove's Modified Dulbecco's Medium (IMDM) |
| Quantity | 10 L |
| Shelf Life | 36 Months |
| Shipping Condition | Room Temperature |
| Classification | Animal Origin-free |
| Culture Type | Mammalian Cell Culture |
| Form | Powder |
| With Additives | Phenol Red |
| Without Additives | No Sodium Bicarbonate |
| pH | 4.4 to 4.8 |
| Unit Size | Each |
Answer:The dry powder formulation is supplied for preparation of 10 L.
Answer:It includes L-glutamine, HEPES, phenol red and sodium pyruvate, but not sodium bicarbonate.
Answer:No. The powder must be reconstituted, supplemented with sodium bicarbonate, pH-adjusted and filtered.
Gibco™ Iscove’s Modified Dulbecco’s Medium (IMDM) powder provides a nutrient-rich basal formulation for culturing selected mammalian and hematopoietic-derived cells. This package prepares 10 L of medium after reconstitution, supplementation and filtration. The specified formulation includes L-glutamine, HEPES, phenol red and sodium pyruvate but excludes sodium bicarbonate, alpha-thioglycerol and 2-mercaptoethanol. It supports the preparation of laboratory culture medium for applications such as cell growth, experimental differentiation and research involving cell lines that require IMDM. Sodium bicarbonate must be added during preparation, with pH adjustment and final filtration before introducing cells. As with other basal media, serum or defined growth supplements depend on the particular model. The dry powder format supports preparation in planned batches while allowing users to add process-specific supplements as required.
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