Thermo Fisher, 42402016, RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine

Name: RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine
Brand: Thermo Fisher
SKU: 42402016

Quantity: 500 mL, Unit Size: Each

Catalog Number: 42402016

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Category

Classical Cell Culture Media

Pack Size500 mL

TagsCell Culture Media Supplements and Reagents Classical and Basal Cell Culture Media RPMI Cell Culture Media

CollectionsThermo Fisher Cell Culture Media & Supplements | Serum-Free, Chemically Defined Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 500 mL
Unit Size: Each
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This RPMI 1640 is modified as follows:
• With:Phenol Red, HEPES
• Without:L-glutamineThe complete formulation is available.Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B_12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.Product UseRPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO_2 environment to maintain physiological pH.

Specifications

General

Concentration	1X
Culture Environment	CO<sub>2</sub>
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Osmolality	270–300 mOsm/kg
Product Line	Gibco
Product Type	RPMI 1640
Quantity	500 mL
Shelf Life	12 Months
Shipping Condition	Room Temperature
Classification	Animal Origin-free
Culture Type	Mammalian Cell Culture
Form	Liquid
Serum Level	Standard Serum Supplementation
Sterility	Sterile-filtered
With Additives	Sodium Bicarbonate
Without Additives	No Sodium Pyruvate
pH	7 to 7.4
Unit Size	Each

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Thermo Fisher, 42402016, RPMI 1640 Medium, low HEPES, low bicarbonate, no glutamine

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