Thermo Fisher, 4309155, SYBR™ Green PCR Master Mix, 1 x 5 mL

Name: SYBR™ Green PCR Master Mix, 1 x 5 mL
Brand: Thermo Fisher
SKU: 4309155

Quantity: 1 x 5 mL, Unit Size: Each, Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

Catalog Number: 4309155

Pricing

Send an RFQ.

View RFQ List

Category

qPCR Master Mixes

Pack Size1 x 5 mL

TagsPCR Equipment and Supplies PCR Supplies Real Time PCR Products Real Time PCR Master Mixes

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 1 x 5 mL
Unit Size: Each
• SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability
• Premixed components stored at 2° to 8°C significantly reduce assay setup timeMaximum Flexibility and Convenience:
• Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
• SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
• The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
• Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
• This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
• SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
• Store at -20°C

Specifications

General

For Use With (Equipment)	7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Format	Tube
No. of Reactions	200 Reactions
Passive Reference Dye	ROX (Pre-mixed)
Polymerase	AmpliTaq Gold DNA Polymerase
Product Line	SYBR
Product Type	Real Time PCR SYBR Master Mix
Quantity	1 x 5 mL
Sample Type	DNA (Genomic), cDNA
Shipping Condition	Wet Ice
Sufficient For	200 Reactions of 50 μL
Volume	1 x 5 mL
Concentration	2X
Detection Method	SYBR
For Use With (Application)	Gene Expression
GC-Rich PCR Performance	High
Label or Dye	SYBR Green
PCR Method	qPCR
Reaction Speed	Standard
Unit Size	Each

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:

[email protected]

Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.

Thermo Fisher, 4309155, SYBR™ Green PCR Master Mix, 1 x 5 mL

Send an RFQ.

General

Notice

More from Thermo Fisher.

NaCl (5 M), RNase-free, 100 mL

Potassium Acetate (3 M), pH 5.5, RNase-free

SDS, 20% Solution, RNase-free

RNase A, DNase and protease-free (10 mg/mL)

More from Thermo Fisher.

NaCl (5 M), RNase-free, 100 mL

Potassium Acetate (3 M), pH 5.5, RNase-free

SDS, 20% Solution, RNase-free

RNase A, DNase and protease-free (10 mg/mL)