Thermo Fisher, 4336974, Matrix Standards Kit, BigDye™ Terminator v3.1, for 31xx/3500/SeqStudio™/SeqStudio™ Flex

Applied Biosystems BigDye Terminator v3.1 Matrix Standards Kit supplies fluorescent reference fragments for spectral calibration on supported capillary-electrophoresis genetic analyzers. The kit includes two tubes of matrix standards; each tube contains a mixture of fluorescently labeled DNA fragments bearing the four BigDye Terminator v3.1 dye colors in 1X TE buffer. The standards establish dye emission profiles used to separate overlapping fluorescence signals during Sanger sequencing analysis. Supported instrument series include 3130/31xx, 3500, SeqStudio and SeqStudio Flex when operated with the appropriate instrument-specific matrix calibration workflow. Mix and briefly centrifuge the standards before preparing any required dilutions, and use the instrument's prescribed calibration and analysis settings. A successful spectral calibration supports accurate differentiation of dye signals; it does not replace sequencing reaction chemistry or run-quality controls. The original matrix standard kit has been discontinued, so confirm the applicability of the current alternative and instrument-specific calibration protocol before procurement.