Thermo Fisher, 4337455, BigDye™ Terminator v3.1 Cycle Sequencing Kit, 100 Reactions
Quantity: 100 Reactions, Unit Size: Each
Catalog Number: 4337455
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Category
Pack Size100 reactions
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| For Use With (Application) | AT-Rich Sequencing (>65%), Comparative Sequencing (Germline Mutations - 50:50), GC-Rich Sequencing (>65%), Mixed-Base Detection, Long-Read Sequencing, Resequencing, De Novo Sequencing - High Throughput (cDNA), GT-Rich or Difficult Template Sequencing |
|---|---|
| For Use With (Equipment) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers |
| Product Line | BigDye™ Terminator |
| Product Type | Sequencing Buffer |
| Quantity | 100 Reactions |
| Technique | Fluorescent Dye Terminator Sequencing |
| Template Compatibility | BAC DNA, Lambda DNA, Genomic DNA (Bacterial), Single Stranded DNA, Rolling Circle Amplified Products, Fosmids, PCR Amplicons (50:50 Heterozygotes), Plasmid DNA (≤15 Kb), PCR Amplicons |
| Format | 96- or 384-well Plate |
| Unit Size | Each |
Answer:It supports de novo sequencing, resequencing and finishing across many common DNA template types.
Answer:This configuration provides reagents for 100 sequencing reactions.
Answer:The fluorescent extension products are separated and detected on compatible capillary-electrophoresis genetic analyzers.
BigDye™ Terminator v3.1 Cycle Sequencing Kit is used for general-purpose dye-terminator Sanger sequencing, including de novo sequencing, resequencing and finishing. The v3.1 chemistry is designed for robust sequence quality across a broad range of templates, including PCR products, plasmids, fosmids, BACs, bacterial genomic DNA, lambda DNA and single-stranded DNA. This configuration provides reagents for 100 sequencing reactions and contains BigDye Terminator v3.1 Ready Reaction Mix, M13 control primer, pREF-BDT control DNA and 5X sequencing buffer. The fluorescent extension products are analyzed on compatible capillary-electrophoresis genetic analyzers. Compared with v1.1 chemistry, v3.1 is commonly selected when longer read performance and broad template flexibility are priorities in routine Sanger sequencing workflows.
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