Thermo Fisher, 4337456, BigDye™ Terminator v3.1 Cycle Sequencing Kit, 1000 Reactions

BigDye™ Terminator v3.1 Cycle Sequencing Kit, 1000 Reactions is designed for Sanger sequencing by fluorescent dye-terminator cycle sequencing. The kit provides premixed reagents that incorporate fluorescently labeled dideoxynucleotide terminators during primer-extension reactions, creating terminated DNA fragments that can be resolved by capillary electrophoresis. This configuration supplies reagents for 1000 sequencing reactions. The kit includes BigDye Terminator Ready Reaction Mix together with the BigDye Terminator v1.1/v3.1 5X Sequencing Buffer, a pGEM-3Zf(+) double-stranded DNA control template and a −21 M13 control primer for reaction and instrument-performance checks. Version 3.1 chemistry is used broadly for routine sequencing of plasmids, PCR products and other DNA templates and is designed for long, high-quality reads when reaction cleanup and electrophoresis conditions are appropriately controlled. The 5X sequencing buffer is formulated specifically for use with the ready-reaction mix, allowing reaction volumes and template conditions to be adjusted while maintaining the ionic environment required by the chemistry. The kit is intended for research use and is used upstream of compatible Applied Biosystems capillary genetic analyzers after post-reaction purification. After cycle sequencing, extension products are normally purified to remove unincorporated dye terminators before capillary analysis. The v3.1 chemistry is optimized for the Applied Biosystems dye set used by compatible genetic analyzers and supports standardized sequencing workflows from reaction setup through electrophoretic detection.