Thermo Fisher, 4351379, TaqMan™ SNP Genotyping Assay, human, S (300 reactions), made to order

Name: TaqMan™ SNP Genotyping Assay, human, S (300 reactions), made to order
Brand: Thermo Fisher
SKU: 4351379

Quantity: S (300 reactions), made to order, Species: Human, Unit Size: Each, • Large selection of SNP assays advances validation and screening studies for disease association and population stratification applications, • Easy-to-use, single-tube format, • Robust assay design provides highly accurate, reproducible and reliable results, • Simple workflow enables quick results

Catalog Number: 4351379

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Category

qPCR Master Mixes

Pack Size0.5 units / pack

TagsPCR Equipment and Supplies PCR Supplies Real Time PCR Products Real Time PCR Assays and Arrays

CollectionsThermo Fisher PCR Equipment & Supplies: Instruments, Master Mixes & Plastics Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: S (300 reactions), made to order
Species: Human
Unit Size: Each
Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5'‐nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). One probe is labeled with VIC dye to detect the Allele 1 sequence; the second probe is labeled with FAM dye to detect the Allele 2 sequence.Our predesigned TaqMan SNP Genotyping Human Assays are a genome-wide collection of millions of human assays, including common 1,000 Genome SNPs, HapMap SNPs, and coding SNPs.Benefits:
• Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
• Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
• Relevant—extensive collection of predesigned human assays offers direct access to content that is relevant to your research
• Tested—all human SNP genotyping assays are functionally tested to ensure allelic discriminationApproximate ship time4–6 days in North America and 6–10 days in EuropeTaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (or another compatible master mix) (sold separately).All assay designs are the product of our bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.All of our predesigned TaqMan Assays are covered by the TaqMan Assays qPCR Guarantee.*Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355)*Terms and conditions apply. For complete details, go to www.thermofisher.com/taqmanguarantee.

Specifications

General

3'Primer Modification	None
5'Primer Modification	None
Description	VIC™ - MGB probe detects Allele 1, FAM - MGB probe detects Allele 2
For Use With (Equipment)	7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ Absolute Q Digital PCR System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex ProFlex PCR System, VeritiPro, SimpliAmp, MiniAmp, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals.
Internal Probe Modification	VIC™ (5'), FAM (5'), MGB (Minor Groove Binder) (3')
No. of Reactions	300 reactions
Product Line	TaqMan
Purification Method	Solid Phase Extraction (SPE)
Purity or Quality Grade	RNase-Free, DNase-Free
Quantity	S (300 reactions), made to order
Shipping Condition	Room Temperature
Species	Human
Target	Over 4.5 million genome-wide human assays are available

FAQs

Question: Which fluorescent labels are used?

Answer:The assay uses FAM and VIC allele-specific fluorescent probes.

Question: How many reactions are included?

Answer:The small-format assay provides approximately 300 reactions at a 25 µL reaction volume.

Question: What chemistry is used for allele discrimination?

Answer:It uses TaqMan 5′-nuclease chemistry with sequence-specific primers and MGB probes.

Application Note

TaqMan™ SNP Genotyping Assay is used to discriminate the two alleles of a specific human single-nucleotide polymorphism by real-time PCR. Each made-to-order assay contains two sequence-specific PCR primers and two allele-specific TaqMan minor-groove-binder probes carrying FAM and VIC fluorescent labels with nonfluorescent quenchers. During amplification, 5′-nuclease cleavage of the correctly hybridized probe releases reporter fluorescence, allowing the instrument to assign genotypes from the relative FAM and VIC signals. This small-format version is supplied as a 40X assay mix and provides approximately 300 reactions at a 25 µL reaction volume. It is suitable for validation studies, disease-association research, population genetics, screening, and other workflows that require targeted SNP genotyping from purified genomic DNA. The single-tube assay format simplifies setup and supports reproducible allele discrimination.

Ordering

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