Thermo Fisher, 4359513, AmpFLSTR™ Yfiler™ PCR Amplification Kit

AmpFLSTR™ Yfiler™ PCR Amplification Kit is used for Y-chromosome STR analysis when male DNA must be characterized in samples that may contain a large background of female DNA. The kit amplifies 17 Y-STR loci in a single PCR reaction and uses a 25 µL reaction format. Its male-specific targets make it useful for forensic casework, sexual-assault evidence and paternity-related investigations where autosomal STR analysis may not resolve a minor male component. The kit supplies PCR reaction mix, Yfiler primer set, control DNA materials, AmpliTaq Gold DNA polymerase and a Yfiler allelic ladder. Amplified fragments are analyzed using compatible genetic analyzers and the appropriate matrix and size-standard system. This 100-reaction configuration supports laboratories running established Yfiler workflows that require reproducible Y-STR profiles from genomic or forensic DNA samples.