Thermo Fisher, 4360788, 7500 Fast Real-Time PCR Systems Spectral Calibration Kit I

7500 Fast Real-Time PCR Systems Spectral Calibration Kit I is designed for spectral calibration of the Applied Biosystems 7500 Fast Real-Time PCR System with a 0.1 mL block. The kit establishes pure fluorescence spectra and multicomponent values for FAM/SYBR Green I, VIC/JOE and NED/TAMRA/ROX dye groups so the instrument can distinguish reporter signals when several dyes are excited and measured during a run. It contains nine preloaded Optical 96-Well Fast Thermocycling Plates: one background plate, seven separate dye-standard plates and one region-of-interest calibration plate. The preloaded and sealed plate format avoids manual preparation of dye standards and provides a controlled optical reference during calibration. The kit is validated specifically for the 7500 Fast instrument configuration rather than QuantStudio systems or standard-block instruments. Store the kit at −15 to −25 °C and protect it from light; the stated shelf life is 12 months from delivery. Spectral calibration is performed during instrument installation, after relevant optical service or when calibration status requires renewal, allowing the software to mathematically separate overlapping fluorescence signals during subsequent qPCR experiments. The background and ROI plates are included because optical calibration requires more than dye spectra alone: the instrument also establishes baseline fluorescence and the imaging region used for the 96-well block. The complete kit therefore supports the full calibration workflow for this instrument format.