Thermo Fisher, 4362201, 7500 Fast Real-Time PCR Systems Spectral Calibration Kit II

7500 Fast Real-Time PCR Systems Spectral Calibration Kit II is designed for extending spectral calibration of the Applied Biosystems 7500 Fast Real-Time PCR System to Cy3, Cy5 and Texas Red fluorescence channels. The kit establishes pure dye spectra and multicomponent values for these three dyes so the instrument can accurately discriminate their signals when they are used in multiplex real-time PCR experiments. It contains three preloaded and sealed Optical 96-Well Fast Thermocycling Plates, with one plate dedicated to each of the Cy3, Cy5 and Texas Red dye standards. The preloaded format reduces handling and eliminates the need to prepare calibration solutions or dispense the dyes into a plate before calibration. This kit complements the standard spectral calibration dye set when assays use these additional reporter channels. It is intended specifically for the 7500 Fast platform and the matching fast 96-well optical geometry. Spectral calibration provides reference profiles used by the instrument software to resolve overlapping fluorescence emission during data collection. The kit is intended for instrument calibration and maintenance rather than for amplification of experimental samples, and it is used as a complete three-plate set during the appropriate calibration procedure. This kit is used when the laboratory intends to detect the additional Cy3, Cy5 or Texas Red channels beyond the primary dye set. Calibration establishes the instrument-specific spectral signatures needed to separate these emissions from neighboring channels during multicolor fluorescence collection.