Thermo Fisher, 4366596, TaqMan™ MicroRNA Reverse Transcription Kit, 200 reactions

TaqMan™ MicroRNA Reverse Transcription Kit provides the components required to convert mature microRNA targets into cDNA for subsequent TaqMan MicroRNA Assays. This configuration supports 200 reverse-transcription reactions and uses MultiScribe™ Reverse Transcriptase. Sequence-specific RT primers supplied with the corresponding TaqMan MicroRNA Assays provide high specificity for mature miRNA rather than precursor forms. The workflow can use approximately 1–10 ng of total RNA for sensitive analysis of limited samples and is designed for a two-step RT-qPCR process. Reverse transcription is typically performed at 42 °C before the cDNA is transferred to a separate real-time PCR reaction with an appropriate TaqMan master mix. The kit is useful for targeted microRNA expression studies where low RNA input and specific mature-miRNA detection are important.