| Calibrated Dye | FAM, JOE, NED, ROX, TAMRA™, VIC, SYBR™ Green |
|---|---|
| Calibration Type | Spectral Calibration |
| Detection Method | Fluorescence |
| For Use With (Application) | Real Time PCR (qPCR) |
| For Use With (Equipment) | Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode |
| Product Line | StepOnePlus |
| Quantity | 1 Kit |
| Shipping Condition | Dry Ice |
| Product Type | Spectral Calibration Kit |
| Unit Size | Each |
Answer:The kit is designed for the StepOnePlus Real-Time PCR System.
Answer:It contains one background plate and two preloaded 96-well Fast optical reaction plates with seven dye standards.
Answer:Store them at -15°C to -25°C and protect them from light.
StepOnePlus™ Real-Time PCR System Spectral Calibration Kit is used to establish dye spectra and multicomponent calibration values for the StepOnePlus real-time PCR instrument. The kit contains one background plate and two preloaded 96-well Fast optical reaction plates. Plate I contains FAM, VIC, NED and ROX dye standards, while Plate II contains SYBR Green I, JOE and TAMRA standards. Running the calibration allows the instrument to distinguish fluorescence from individual dyes in multiplex and singleplex assays. The kit is intended for instrument maintenance, optical calibration and restoration of spectral performance after service or when calibration is required by the workflow. Reagents are shipped on dry ice and should be stored at -15°C to -25°C protected from light until use.
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